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Cloning and Expression
Transfection Kits and Reagents
Transfection Reagents
ViaFect Transfection Reagent
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ViaFect Transfection Reagent
ViaFect Transfection Reagent
ViaFect Transfection Reagent from Promega
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Citations:
(134)
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ViaFect Transfection Reagent
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Description
ViaFect Transfection Reagent is a novel formulation reagent designed to efficiently introduce DNA into a wide variety of cell lines
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
ViaFect Transfection Reagent
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
E4982
Quantity
2 x 0.75ml
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (134)
(81)
Generation of PAX7 Reporter Cells to Investigate Skeletal Myogenesis from Human Pluripotent Stem Cells
STAR protocols
December 18, 2020
Xi H, Young CS, Pyle AD.
Primary Cell 4D-Nucleofector X Kit L,
Lonza
,Cat#:
V4XP-3024
ViaFect Transfection Reagent
,
Promega
,Cat#:
E4981
SURVEYOR Mutation Detection Kit for Standard
Gel Electrophoresis
,Integrated DNA Technologies,Cat#:
(82)
The mitotic protein NuMA plays a spindle-independent role in nuclear formation and mechanics
The Journal of cell biology
December 7, 2020
Serra-Marques A, Houtekamer R, Hintzen D, Canty JT, Yildiz A, Dumont S.
E and F, NuMA-FL-EGFP only), inducible NuMA KO RPE1 cells were
transfected
with DNA using ViaFect (
E4981
;
Promega
) according to the manufacturer’s instructions 48 h before imaging. For rescue experiments (Fig.
(83)
Aster-B coordinates with Arf1 to regulate mitochondrial cholesterol transport
Molecular metabolism
December 1, 2020
Andersen JP, Zhang J, Sun H, Liu X, Liu J, Nie J, Shi Y.
Invitrogen
). 2.3
Cell transfection
and
confocal
analysis C2C12
transfections
were performed using Viafect (
E4981
) from
Promega
. Cells were seeded overnight in 6-cm culture dishes and then
transfected
with 2.5 μg of
(84)
PICH regulates the abundance and localization of SUMOylated proteins on mitotic chromosomes
Molecular biology of the cell
November 1, 2020
Hassebroek VA, Park H, Pandey N, Lerbakken BT, Aksenova V, Arnaoutov A, Dasso M, Azuma Y.
into the genome. DLD-1 cells were
transfected
with guide plasmids and donor plasmid using ViaFect (#
E4981
,
Promega
) on 3.5 cm dishes. The cells were split and replated on 10 cm dishes at ∼20% confluency; 2
(85)
A Small-Molecule Approach to Restore a Slow-Oxidative Phenotype and Defective CaMKIIβ Signaling in Limb Girdle Muscular Dystrophy
Cell Reports Medicine
October 20, 2020
Jian Liu, Jesus Campagna, Varghese John, Robert Damoiseaux, Ekaterina Mokhonova, Diana Becerra, Huan Meng, Elizabeth M. McNally, April D. Pyle, Irina Kramerova,
et al.
Inhibitor Cocktail,Sigma,Cat# P8340 DMSO,
Sigma
,Cat#
D2438
ViaFect Transfection Reagent
,
Promega
,Cat#
E4981
Ponceau S Red,Sigma,Cat# P7170 iScript Reverse Transcriptase Supermix,
Bio-Rad
,Cat. No.:
1708841
,, Critical
(86)
Soluble RARRES1 induces podocyte apoptosis to promote glomerular disease progression
The Journal of clinical investigation
October 1, 2020
Chen A, Feng Y, Lai H, Ju W, Li Z, Li Y, Wang A, Hong Q, Zhong F, Wei C,
et al
.
plasmids were
transfected
to
human
podocyte
cell lines with
ViaFect transfection reagent
(
Promega
,
E4981
). At 36 hours after
transfection
, the cells were harvested and total protein was extracted from the
(87)
Inhibition of focal adhesion kinase increases myofibril viscosity in cardiac myocytes
Cytoskeleton (Hoboken)
September 9, 2020
Nilay Taneja, Matthew R. Bersi, Megan L. Rasmussen, Vivian Gama, W. David Merryman, Dylan T. Burnette
as recommended by the manufacturer. Exogenous expression was performed using Viafect (
Promega
, Cat.
E4981
). 200 ng of plasmid DNA was used to
transfect
one well of a 96-well culture plate according to the manufacturer’s
(88)
SCYL1 arginine methylation by PRMT1 is essential for neurite outgrowth via Golgi morphogenesis
Molecular biology of the cell
August 15, 2020
Amano G, Matsuzaki S, Mori Y, Miyoshi K, Han S, Shikada S, Takamura H, Yoshimura T, Katayama T.
were
transfected
at 10 DIV with pEGFP-C1 (Clontech, Palo Alto, CA) using
ViaFect transfection reagent
(
E4981
,
Promega
, Madison, WI) according to the manufacturer’s protocol. At 4 h after
transfection
, the medium
(89)
Transfection of hPSC-Cardiomyocytes Using Viafect™ Transfection Reagent
Methods and Protocols
August 9, 2020
Sara E. Bodbin, Chris Denning, Diogo Mosqueira
Loughborough, UK, Cat. No: 11573397) Viafect™ (
Promega
, Southampton, UK, Cat. No:
E4981
1X 0.75 mL,
E4982
2X 0.75 mL) Opti-
MEM
™ (
Gibco
™,
Thermofisher
, Loughborough, UK, Cat. No:
31985070
) Axygen™ MaxyClear
(90)
A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells
Cell stem cell
July 2, 2020
Xi H, Langerman J, Sabri S, Chien P, Young CS, Younesi S, Hicks M, Gonzalez K, Fujiwara W, Marzi J,
et al
.
Primary Cell 4D-Nucleofector X Kit L,
Lonza
,Cat#:
V4XP-3024
ViaFect Transfection Reagent
,
Promega
,Cat#:
E4981
Deposited Data,Deposited Data,Deposited Data Raw
sequencing
reads and processed DGE matrices,This
(91)
Coupling to substrate adhesions drives the maturation of muscle stress fibers into myofibrils within cardiomyocytes
Molecular Biology of the Cell
June 1, 2020
Nilay Taneja, Abigail C. Neininger, Dylan T. Burnette
mounting.
Protein expression
For
protein expression
in hiCMs, 200 ng plasmid and 0.4 µl ViaFect (
E4981
,
Promega
, Madison WI) were added to a total of 10 µl of Opti-
MEM
(
ThermoFisher
, Waltham, MA) and added
(92)
Reversible promoter methylation determines fluctuating expression of acute phase proteins
eLife
March 30, 2020
Shi-Chao Zhang, Ming-Yu Wang, Jun-Rui Feng, Yue Chang, Shang-Rong Ji, Yi Wu
transfected
into Hep3B cells with
ViaFect Transfection Reagent
(
Promega
, Madison, WI; catalog number:
E4982
; lot number: 0000251076, 0000136819). 48 hr later,
gene expression
was determined with q-
PCR
. For Cas9-mediated
(93)
PRMT1-mediated methylation of the microprocessor-associated proteins regulates microRNA biogenesis
Nucleic acids research
January 10, 2020
Spadotto V, Giambruno R, Massignani E, Mihailovich M, Maniaci M, Patuzzo F, Ghini F, Nicassio F, Bonaldi T.
the pOG44 Flp-Recombinase (Thermo Fisher Scientific) using the
ViaFect transfection reagent
(
Promega
,
E4981
). After
transfection
, cells were growth in medium supplemented with 1μg/mL Blasticidine S hydrochloriode
(94)
Knockout of
EGFL6
by CRISPR/Cas9 Mediated Inhibition of Tumor Angiogenesis in Ovarian Cancer
Frontiers in oncology
January 1, 2020
Zhu W, Liu C, Lu T, Zhang Y, Zhang S, Chen Q, Deng N.
psPAX2 and pMD2G were co-
transfected
into HEK293T by
ViaFect™ Transfection Reagent
(
Promega
, USA, Cat:
E4982
).
Lentivirus
was harvested at 48 h. The 2-ml
lentivirus
solution with 8 μg/ml polybrene (Solarbio, Cat:
(95)
TAK1 suppresses RIPK1-dependent cell death and is associated with disease progression in melanoma
Cell death and differentiation
December 1, 2019
Podder B, Guttà C, Rožanc J, Gerlach E, Feoktistova M, Panayotova-Dimitrova D, Alexopoulos LG, Leverkus M, Rehm M.
described previously [18]. Cells were
transfected
by using ViaFectTM
transfection reagent
(Cat no:
E4981
;
Promega Corporation
, Madison, USA) according to the manufacturer’s instructions. Cells were washed
(96)
PODXL1 promotes metastasis of the pancreatic ductal adenocarcinoma by activating the C5aR/C5a axis from the tumor microenvironment
Neoplasia (New York, N.Y.)
December 1, 2019
Saito K, Iioka H, Maruyama S, Sumardika IW, Sakaguchi M, Kondo E.
The hCas9 and PODXL1 gRNA vector were cotransfected into cells using
ViaFect™ Transfection Reagent
(#
E4981
,
Promega
, Madison, WI). Twenty-four hours posttransfection, the cells were cultured with RPMI medium
(97)
Live Cell Membranome cDNA Screen: A Novel Homogenous Live Cell Binding Assay to Study Membrane Protein-Ligand Interaction
SLAS Discovery
December 1, 2019
Xun Shen et al.
penicillin/ streptomycin (
15070063
,
ThermoFisher Scientific
). For reverse
transfection
,
ViaFect transfection reagent
(
E4981
,
Promega
, Madison, WI) was mixed with
Opti-MEM I reduced serum medium
(31985,
Gibco
) at ratio of
1:50
.
(98)
Biosensors Show the Pharmacokinetics of S-Ketamine in the Endoplasmic Reticulum
Frontiers in Cellular Neuroscience
November 12, 2019
Kallol Bera et al.
DopaNeurons were
transfected
after either 13 or 21 days in culture using the Viafect kit (
Promega
, Cat. #
E4981
) at 4:1
transfection reagent
(µl): DNA (µg) ratio. The
transfection
mixture was prepared in 100 µl OptiMEM
(99)
Poxviruses as Gene Therapy Vectors: Generating Poxviral Vectors Expressing Therapeutic Transgenes
Methods in Molecular Biology
November 6, 2019
Steven J. Conrad, Jia Liu
agarose. 8. PBS, with calcium and magnesium, sterile. 9.
ViaFect Transfection Reagent
,
Promega
(catalog
E4982
). Consumables 1. 150 mm cell culture dish, treated, DNase/RNase free, sterile. 2. 24 well
tissue culture
(100)
Mammalian kinetochores count attached microtubules in a sensitive and switch-like manner
The Journal of cell biology
November 4, 2019
Kuhn J, Dumont S.
University, Tallahassee, FL) was swapped for EGFP in
Hec1
-9D-EGFP (gift from J. DeLuca) using ViaFect (
E4981
;
Promega
) 48 h (HeLa) or 72 h (PtK2) before imaging. For siRNA
knockdown
, cells were treated with 100
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Reviews
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(1)
6/17/2019
Easy-to-Use Transfection Reagent with Good Results from Promega
Wei Yang Sit
Highly recommended transfection reagent for those who intend to carry out transfection.
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ViaFect Transfection Reagent from Promega
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